Table 1.
Main characteristics of antibodies used for the immunohistochemical (IHC) stains (GIST, gastrointestinal stromal tumor)
| Antibody (company) | Clone | Dilution | Antigen retrieval | Positive control |
|---|---|---|---|---|
| Maspin (Novocastra, Newcastle-upon-Tyne, UK) | EAW24 | 1:25 | Incubation with citrate buffer (pH 6.0) - 60 minutes at 100°C | • external - renal tubes |
| • internal - NS | ||||
| CD31/PECAM-1 (LabVision, Fremont, CA, USA) | JC/70A | 1:25 | Incubation with high pH-solution (pH 10.0) - 30 minutes at 100°C | • external - placenta |
| • internal - blood vessels - endothelial cells | ||||
| VEGF-A (LabVision) | VG1 | 1:50 | Incubation with high pH-solution (pH 10.0) - 30 minutes at 100°C | • external - renal tubes |
| • internal - normal mature vessels - endothelial cells | ||||
| COX-2 (Novocastra) | monoclonal | 1:100 | Incubation with citrate buffer (pH 6.0) - 60 minutes at 100°C | • external - brain |
| • internal - lymphocytes | ||||
| CD34 (Dako Glostrup, Denmark) | Qbend/10 | 1:100 | Incubation with high pH-solution (pH 10.0) - 30 minutes at 100°C | • external - placenta |
| • internal - blood vessels - endothelial cells | ||||
| DOG-1 (Novocastra) | K9 | 1:50 | Incubation with citrate buffer (pH 6.0) - 60 minutes at 100°C | • external - renal tubes |
| • internal - NS | ||||
| CD117 (Dako) | polyclonal rabbit | 1:500 | Incubation with high pH-solution (pH 10.0) - 30 minutes at 100°C | • external - intestine |
| • internal - NS |