Skip to main content
. 2015 Apr 1;6:6765. doi: 10.1038/ncomms7765

Figure 7. Ubiquitination of GSK3β is required for the formation of a signalling complex containing TRIF, TRAF6, TAK1 and GSK3β.

Figure 7

(a–c) The GSK3β K183R mutant abrogates the interaction of GSK3β with TRAF6 but not with TRIF or TAK1. HEK293T cells were transfected with the indicated combinations of expression plasmids. Co-imunoprecipitations were performed with an anti-HA antibody followed by western blotting with the indicated antibodies. The expression levels of the transfected plasmids were confirmed by western blotting of whole-cell lysates. (d,e) The GSK3β K183R mutation disrupts the TRIF–GSK3β–TRAF6–TAK1 complex. HEK293T cells were transfected with the indicated combinations of expression plasmids. Co-imunoprecipitations were performed with anti-HA (d) or with anti-Flag (e) antibodies followed by western blotting with the indicated antibodies. The expression levels of the transfected plasmids were confirmed by western blotting of whole-cell lysates. Data are representative of two or three independent experiments.