Table 2.
Relative abundance (percentage) of rRNA OTUs and overlap at 97% similarity between metagenome (MG) and metatranscriptome (MT) reconstructed OTUs and isolated bacteria.
| Sequence type | Sample | Treatmenta | Library prep.b | Number of EMIRGE OTUs | Relative Abundancec (Domains) | Relative Abundance (Bacteria) | Relative abundance of isolates with >97 similarity to MG/MT reconstructed sequences | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Bacteria (%) | Archaea (%) | Eukaryota (%) | M. vaginatus (%) | Cyanobacteria (non M. vaginatus) (%) | Non-cyanobacteria (%) | Number | Total Bacteria (%) | Non-phototrophic Bacteria (%) | |||||
| Metagenome | MTG | Prior wet-up | Total DNA | 251 | 99.43 | 0.57 | BDL | 41.34 | 6.69 | 51.97 | 8 | 4.28% | 8.23% |
| Metatranscriptome | 6 | Night time (18 h) | Total RNA | 4708 | 98.03 | 0.26 | 1.71% | 16.96 | 34.80 | 48.25 | 16 | 1.55% | 3.21% |
| DSN treated | 3829 | 92.82 | 0.58 | 6.60% | 3.23 | 6.41 | 90.36 | 12 | 2.98% | 3.30% | |||
| 9 | Day time (25.5 h) | Total RNA | 50 | 99.75 | 0.25 | BDL | 24.59 | 58.15 | 17.26 | 2 | 0.75% | 4.37% | |
| DSN treated | 213 | 99.92 | 0.08 | BDL | 9.67 | 19.54 | 70.79 | 1 | BDL | BDL | |||
a
The metagenomic library was prepared with DNA extracted from samples Prior wet-up, and the metatranscriptomic libraries were prepared with RNA extracted 18 h (night time) or 25.5 h (day time) after wet-up.
b
RNA used for library preparation. The libraries were prepared with total cDNA (Total RNA) or Double Strand Specific Nuclease (DSN) treated cDNA to reduce the influence of dominant organisms (DSN treated).
c
BDL, below detection limit.