Figure 2.

BMS-536924 induces apoptosis of both TMZ-sensitive and -resistant glioma cells.

Notes: (A) TMZ-resistant and -sensitive cells were treated with BMS-536924 (100~800 nM) for 48 hours, followed by PI staining and flow cytometry analysis. (B) TMZ-resistant and -sensitive cells were incubated with BMS-536924 (100~800 nM) for 48 hours. The nuclei were stained with DAPI and analyzed using a fluorescent microscope. The representative images are shown (the fragmented nuclei were labeled by arrow heads). (C) The number of cells with condensed/fragmented nuclei was quantitated by counting in five random fields, and the inhibition was calculated.

Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; PI, propidium iodide; TMZ, temozolomide.