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. 2015 Feb 23;125(4):1396–1400. doi: 10.1172/JCI71081

Figure 3. TLR4 signaling is impaired in macrophages from 3BP2-deficient and cherubic mice.

Figure 3

(A) Lysates from Sh3bp2WT/WT BMM were immunoprecipitated with protein G–sepharose beads coated with control Abs, anti-3BP2 Abs, or no Abs and immunoblotted using the indicated Abs. (B and C) Immunoblot analysis and (D) measurement of RAC activity by G-LISA were performed on cell lysates of BMM stimulated with LPS for the indicated times. (E) LPS-stimulated BMM were stained with fluorescent phalloidin and analyzed by flow cytometry (n = 3). Representative microscopic pictures of phalloidin and DAPI-stained BMM, either untreated (left) or stimulated with LPS for 30 minutes (right), are shown. (F) TNF-α was measured by ELISA in the serum of untreated mice (n = 6). (G) LPS was added for 28 days in mouse drinking water, and TNF-α in the serum was measured by ELISA (n = 6). P values were determined by the following tests: 2-way ANOVA (D, E and G); (Kruskal-Wallis test (F). *P < 0.05; **P < 0.01; ***P < 0.001.