Table 1.

Polymorphic genes involved in aliphatic GSL synthesis and activation

DOI: http://dx.doi.org/10.7554/eLife.05604.021

Gene name	Locus	ATG #	Gene type	Gene function	Mutation type in Col-0
MYB28	MYB28	At5g61420	TF	Positive regulator of aliphatic GSL (Sønderby et al., 2007, 2010)	T-DNA
MYB29	MYB29	At5g07690	TF	Positive regulator of aliphatic GSL (Sønderby et al., 2007, 2010)	T-DNA
MAM1	MAM1	At5g23010	Enzyme	Controls 3C–4C chain elongation (Haughn et al., 1991; de Quiros et al., 2000; Kroymann et al., 2003)	EMS
GSOX1	GSOX	At1g65860	Enzyme	Converts MT to MSO GSL (Hansen et al., 2007; Li et al., 2008)	T-DNA
GSOX3	GSOX	At1g62560	Enzyme	Converts MT to MSO GSL (Hansen et al., 2007; Li et al., 2008)	T-DNA
AOP2	AOP	At4g03060	Enzyme	Converts MSO to alkenyl GSL (Kliebenstein et al., 2001c)	35S OX
AOP3	AOP	At4g03050	Enzyme	Converts 3MSO to hydroxy-propyl GSL (Kliebenstein et al., 2001c)	n/a
GSOH	GSOH	At2g25450	Enzyme	Converts butenyl to OHB (Hansen et al., 2008)	T-DNA
ESP	ESP	At1g54040	Co-factor	Guides formation of activated GSL to nitriles (Lambrix et al., 2001)	35S OX

Shown are the identities, functions and mutation types of nine genes representing seven loci important for aliphatic GSL synthesis and activation. These genes were chosen for mutant laboratory population of Arabidopsis thaliana accession Col-0 due to the fact that they represent the majority of aliphatic GSL variation observed in Arabidopsis. Each of these genes is naturally polymorphic among Arabidopsis accessions.