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. 2015 Apr 13;4:e05604. doi: 10.7554/eLife.05604

Table 6.

Mixed model of fitness phenotypes in the field

DOI: http://dx.doi.org/10.7554/eLife.05604.036

Fixed effects Absolute fitness (w/survivorship) Absolute fitness (w/out survivorship)
Source of variation df SS MS F p value df SS MS F p value
Genotype 16 455453 28466 2.2 4.9E-03 16 397186 24824 2.3 2.0E-03
Environment 2 88326 44163 2.2 2 127177 63588 4.6
Treatment 1 1948 1948 0.2 1 2042 2042 0.3
Geno:Env 32 706781 22087 1.7 0.01 32 508962 15905 1.5 0.04
Geno:Trt 16 137795 8612 0.6 16 169036 10565 1.0
Env:Trt 2 2918 1459 0.1 2 4883 2442 0.2
Geno:Env:Trt 32 348291 10884 0.8 32 235224 7351 0.7
Random Effects Absolute fitness (w/survivorship) Absolute fitness (w/out survivorship)
Source of variation df SS MS Chi.sq p value df SS MS Chi.sq p value
Plot(Trt:Env) 1 2640 2640 216.5 0 1 3311 3311 279.3 0
Residual 1692 12581 7 NA 1451 10061 7 NA
Fixed effects Relative fitness Survivorship
Source of variation df SS MS F p value df SS MS F p value
Genotype 16 28 2 2.7 3.9E-04 16 5 0 4.9 6.9E-10
Environment 2 2 1 1.0 2 2 1 9.3 0.01
Treatment 1 0 0 0.2 1 0 0 1.2
Geno:Env 32 39 1 1.8 4.2E-03 32 9 0 3.8 3.8E-12
Geno:Trt 16 8 1 0.8 16 1 0 0.8
Env:Trt 2 0 0 0.1 2 0 0 0.1
Geno:Env:Trt 32 17 1 0.8 32 3 0 1.1
Random effects Relative fitness Survivorship
Source of variation df SS MS Chi.sq p value df SS MS Chi.sq p value
Plot(Trt:Env) 1 0.1 0.1 184.5 0 1 0 0 0
Residual 1692 1 3.8E-04 NA 1900 0.1 3.5E-05 NA

A linear mixed model was fitted to phenotypes measured on plants grown in the field. Variation was partitioned among the fixed effects, Genotype, Environment, and Treatment as well as a random factor, Plot, inside which Treatment and Environment were nested. Phenotypic data used in the model was collected on 17 genotypes from three environments and two treatment groups. df = degrees of freedom, SS = Type II Sums of Squares variation, MS = Mean Squared variation, F = F statistic. Non-significant p values (>0.05) are represented by a dash.