Figure 1.

Der p effect on T cell CysLT₁ and CysLT₂ mRNA and protein expression. Comparison of CysLT₁ and CysLT₂ mRNA expression in CD4⁺ and CD8⁺ T cells from healthy controls and HDM-allergic (HDMA) patients following stimulation with the Der p allergen (200 AU/mL). PBMC from healthy control and HDMA subjects were cultured for 48 h (qPCR) or 72 h (FACS) in the presence of glycerol vehicle or Der p before CD4⁺ and CD8⁺ T cells were purified and collected for analysis. CysLT₁ (a) and CysLT₂ (b) mRNA expression was measured by real-time quantitative PCR analysis. Data are presented as fold (ΔΔCt) increases over GAPDH mRNA (±SEM). ^∗ P < 0.05 and ^∗∗ P < 0.01, relative to vehicle glycerol; n = 6 for controls; n = 10 for HDMA. Cell surface expression of CysLT₁ (c) receptor was evaluated using rabbit polyclonal anti-CysLT₁ receptor Ab, followed by labeling with FITC-conjugated goat anti-rabbit IgG. Cells were further incubated with anti-CD4 PE-Cy5 and anti-CD8 PE Ab before analysis on a FACSCalibur flow cytometer. Data are expressed as geometric mean (±SEM) fluorescence intensity (MFI). ^∗ P < 0.05; n = 6 for controls; n = 10 for HDMA.