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. 2015 Mar 14;17(1):60. doi: 10.1186/s13075-015-0569-6

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© Arkesteijn et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Notochordal cells (NCs) and nucleus pulposus cells (NPCs) in coculture (control: articular chondrocytes (ACs)). Depiction of the (A) DNA content and (B) glycosaminoglycan (GAG) content normalized to DNA (GAG/DNA) and the relative gene expression of (C) NC marker brachyury; (D) aggrecan; (E) collagen, type II, α1 (collagen 2A1); and (F) collagen, type I, α1 (collagen 1A1). White bar = day 1, gray bar = day 15, black bar = day 28. ^$ P < 0.05, significant difference in the increase in gene expression in time between groups. ^# P < 0.001, significant difference in the increase in gene expression in time between groups. Only relevant comparisons are displayed.