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. 2015 Apr 7;8:735–744. doi: 10.2147/OTT.S80945

Figure 3.

Figure 3

Secretory miR-27a induced liver cancer cell G1/S transition by targeting FOXO1.

Notes: (A) Flow cytometric analysis of the cell cycle of HepG2 cells alone, under cocultured with 3T3-L1, miR-27a overexpressing 3T3-L1 and miR-27a overexpressing 3T3-L1 plus miR-27a inhibitor for 48 hours. (B) qRT-PCR analysis of FOXO1, p21, p27, and cyclin D1 mRNA expression in HepG2 cells alone, under cocultured with 3T3-L1, miR-27a overexpressing 3T3-L1 and miR-27a overexpressing 3T3-L1 plus miR-27a inhibitor for 48 hours. (C–D) Western blot analysis of FOXO1, p21, p27, and cyclin D1 protein expression in HepG2 cells alone, under cocultured with 3T3-L1, miR-27a overexpressing 3T3-L1 and miR-27a overexpressing 3T3-L1 plus miR-27a inhibitor for 48 hours. Each bar represents the mean ± SD of three independent experiments. *P<0.05 compared with control, **P<0.05 compared with miR-27a overexpressed 3T3-L1 group.

Abbreviations: qRT-PCR, quantitative real-time polymerase chain reaction; FOXO1, forkhead box O1; p21, cyclin-dependent kinase inhibitor 1A; p27, cyclin-dependent kinase inhibitor 1B; SD, standard deviation.