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. Author manuscript; available in PMC: 2015 Apr 14.
Published in final edited form as: J Gastrointest Surg. 2008 Jul 30;12(11):1938–1950. doi: 10.1007/s11605-008-0581-0

Figure 8.

Figure 8

Both in vitro and in vivo photobleaching were compared for AlexaFluor 488- versus Oregon Green-labeled cells. In vitro Alexa-Fluor 488- and Oregon Green-stained ASPC-1 cells (a) were exposed to bright OR lighting for 24 h. The cells were imaged on a fluorescence microscope at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, and 24 h. The in vitro fluorescence signal in Oregon Green-labeled cells decreased by 50% and 67% at 9 and 24 h, respectively. AlexaFluor 488-stained cells lost only 10% and 45% of their signal at 9 and 24 h. In vivo AlexaFluor 488- and Oregon Green-stained subcutaneous ASPC-1 tumors (b) were exposed to bright OR lighting for 8 h. The tumors were imaged on the OV100 at 0, 2, 4, 6, and 8 h. The in vivo fluorescence signal in Oregon Green-labeled tumors decreased by about 20% over 8 h, whereas that of the AlexaFluor 488-labeled tumors decreased by only 10%; n= 3 AlexaFluor 488, n=3 Oregon Green.