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Presence of human- or mouse-derived microbiota increases tissue concentration of 5-HT and Chga mRNA in the mouse proximal colon. A) 5-HT concentration was assessed in tissue lysates from the proximal colon by ELISA [8 mice per group (4 males)]. One-way ANOVA: *P < 0.05; ***P < 0.001. B) Relative expression of Chga mRNA (Chga, a neuroendocrine secretory marker) was determined in the proximal colon by qRT-PCR (14–16 mice per group). One-way ANOVA: *P < 0.05; ***P < 0.001.
