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. 2014 Dec 4;29(4):1185–1197. doi: 10.1096/fj.14-260570

Figure 5.

Figure 5.

PON3 is localized in MAM and influences mitochondrial function. A) Distribution of PON3 protein in various organelles isolated from the WT and Pon3KO livers. CM, crude mitochondria; ER, endoplasmic reticulum; MAM, mitochondria-associated membrane; PM, pure mitochondria; T, total lysate. Distribution patterns of calnexin, VDAC, and cytochrome c are also shown. B, C) Complex II–IV-dependent respiration of mitochondria isolated from the liver of WT and Pon3KO mice fed a chow diet. D–F) Mice were maintained on the CC diet for 10 wk before liver samples were collected for determination of (D) mitochondrial superoxide levels, (E) mitochondrial complex II + III activity, and (F) extent of apoptosis as measured by TUNEL assay. *P < 0.05, ***P < 0.001 between the 2 genotype groups.