TABLE 3.
Drosophila nonmuscle myosin-2 | Drosophila nonmuscle myosin-2 M466I | Acanthamoeba nonmuscle myosin-2 | Dictyostelium nonmuscle myosin-2 | Human nonmuscle myosin-2A | Blebbistatin interaction | |
---|---|---|---|---|---|---|
IC50 (µM) | >>200 | 36.3 | 83 (14) | 4.9 (14) | 5.1 (14) | |
Residue | Leu271 | Leu271 | Leu262 | Leu262 | Leu258 | Benzyl ring |
Residue | Phe477 | Phe477 | Phe468 | Phe466 | Phe464 | Benzyl ring |
Residue | Glu478 | Glu478 | Glu469 | Glu467 | Glu465 | Benzyl ring |
Residue | Val651 | Val651 | Val652 | Val630 | Val646 | Benzyl ring |
Residuea | Ala467 | Ala467 | Ser458 | Ser456 | Ala454 | Tetrahydropyrrolo ring |
Residue | Ile482 | Ile482 | Ile473 | Ile471 | Ile469 | Tetrahydropyrrolo ring |
Residue | Thr485 | Thr485 | Thr476 | Thr474 | Thr472 | Methylquinolinone |
Residue | Tyr655 | Tyr655 | Tyr656 | Tyr634 | Tyr650 | Methylquinolinone |
Residue | Gln658 | Gln658 | Gln659 | Gln637 | Gln653 | Methylquinolinone |
Residue | Leu662 | Leu662 | Leu663 | Leu641 | Leu657 | Methylquinolinone |
Residuea | Met466 | Ile466 | Ile459 | Ile457 | Ile455 | None |
Reference binding site residues were previously described for the Dictyostelium nonmuscle myosin-2/blebbistatin complex by Allingham et al. (29). Residues corresponding to Drosophila nonmuscle myosin-2 Met466 are listed for completeness. aDeviations in the amino acid sequence.