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. 2014 Dec 9;29(4):1344–1359. doi: 10.1096/fj.14-265512

Figure 5.

Figure 5.

Reciprocal feedback regulation of Bglap2 and Saa3. A) Treatment of 10-d-old MC3T3-E1 matured osteoblasts with recSaa3 for a further 3 d significantly decreases the mRNA expression of the osteoblastic marker Bglap2. B) In MLO-A5 preosteocytes, knockdown of Saa3 expression (Saa3 expression is as in Fig. 4A, C) provokes a significant up-regulation of Bglap2 levels. C) In MLO-Y4 cells, Bglap2 treatment decreases Saa3 expression in a concentration (conc.)-dependent manner. 18S rRNA is a reference gene for RT-qPCR analysis. Control (Ctrl) or negative control (siNeg Ctrl) is set to 1, and control (Ctrl) or treated probes are referred to as fold change to Ctrl or siNeg Ctrl. Values are represented as the mean ± sd; for all experiments n = 3. *P ≤ 0.05; **P ≤ 0.01.