Figure 4.

Ischemia-induced macrophage infiltration is increased and long-lasting in young p75KO mice. Representative ×20 bright field microscopy images for immunostaining with macrophage marker: F4/80- (brown) and hematoxylin-stained nuclei (blue) in HL muscle tissue from WT and p75KO mice, respectively at (A, F) presurgery control, (B, G) d 1 after HLI surgery, (C, H) d 3 after HLI surgery, (D, I) d 7 after HLI surgery, and (E, J) d 10 after HLI surgery. K) Graphic representation of the number of F4/80⁺ cells in the HL muscle tissue isolated from WT and p75KO mice before surgery and up to 10 d after HLI surgery. All results are presented as means ± sem; n = 3–5 animals per time point per group for WT (solid blue line) and p75KO (solid red line) groups. Statistical significance was assigned when P < 0.05.