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. 2014 Dec 30;29(4):1467–1479. doi: 10.1096/fj.14-267054

Figure 8.

Figure 8.

A) miR-429 modulates VEGFA expression indirectly through changing HIF1A levels. HUVECs were transfected with miR-429 analog or inhibitor, and the mRNA levels were monitored in quantitative real-time PCR experiments in normoxic conditions and after 4 and 8 hours of hypoxia. The mRNA level results from 3 independent experiments (n = 12) are normalized to 18S rRNA levels and expressed as a fold change over the transfection control. The predicted target site of miR-429 in VEGFA 3′ UTRs is shown on the right. The VEGFA (B) and HIF1A (C) mRNA levels were monitored in quantitative real-time PCR experiments following transfection of HUVECs with VEGFA TP or HIF1A TP and/or miR-429 analog (Mimic 429). The mRNA level results from 3 independent experiments (n = 12) are normalized to 18S rRNA levels and expressed as a fold change over the transfection control. *P < 0.05.