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. 2015 Jan 8;29(4):1551–1563. doi: 10.1096/fj.14-260828

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Figure 7. — AQP1 deficiency promotes cyst development in embryonic kidney cyst model. A) Western blot of the embryonic kidneys from wild-type mice on embryonic day 12.5, 13.5, 15.5, 17.5, and at age 8 wk. B) Light micrographs of embryonic kidney cultures in transwells maintained for 6 days. Embryonic day 13.5 kidneys were exposed to 0 (control) or 100 μM 8-Br-cAMP. Each series of photographs shows the same kidney on successive days in culture. C) Percentage cyst area in AQP1^+/− and AQP1^−/− embryonic kidneys on day 6 in culture. Means ± sem; n = 6–10, *P < 0.05 compared with AQP1^+/− kidneys. D) AQP1 and LTL immunofluorescence control and 8-Br-cAMP-treated embryonic kidneys on day 6 in culture.