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. 2015 Apr 10;16(1):281. doi: 10.1186/s12864-015-1480-x

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© Khalil et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Validation of the silencing of two rye genes in triticale. Two rye coding sequences that were not expressed in triticale but found to be present in the genomic DNA of triticale were rescreened by RT-PCR on the cDNA generated from RNAs of two-week old rye, triticale and wheat seedling. Ubiquitin gene was used as a control for the expression in all plants. (A) RT-PCR revealed that R8 from the rye genome was silenced in triticale shoot. (B) Low expression of R29 from the rye genome was detected in triticale root. The ladder has DNA fragments with molecular sizes of 1500 bp, 1000 bp, 750 bp, and 500 bp.