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. 2014 Nov 24;20:50. doi: 10.1186/1678-9199-20-50

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© Pinto et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Evaluation of ultrastructural damage by transmission electron microscopy (TEM). Promastigotes of L. (L.) infantum were incubated with SaFr1 at different times: (A) control (untreated); (B) one-hour incubation; (C) two-hour incubation; (D) and (E) four-hour incubation and (F) 16-hour incubation. m: mitochondrial damage; v: vacuole; pm: plasma membrane integrity. Bar represents (A) 0.5 μm; (B) 1 μm; (C) 0.1 μm; (D) 0.5 μm; (E) 100 nm; (F) 100 nm.