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. 2014 Nov 24;20:50. doi: 10.1186/1678-9199-20-50

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© Pinto et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Evaluation of the plasma membrane permeability of L. (L.) infantum incubated with SaFr1 by SYTOX Green®. The fluorescence intensity was determined using a fluorimetric microplate reader with excitation and emission wavelengths of 485 and 520 nm, respectively. Triton X-100 was used as the positive control of the assay.