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. 2015 Apr 8;14:77. doi: 10.1186/s12943-015-0351-z

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© Koizume et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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cDNA microarray analysis of genes activated by CoCl ₂ stimulation in an Sp1-dependent manner. a) western blot analysis of lysates from cells transfected with non-specific (NS) or Sp1 siRNA. b) log2 ratio of mRNA levels transfected with NS- or Sp1-siRNAs and then cultured for 24 h. Cells were further cultured for an additional 4 h under stimulation with CoCl₂ (500 μM) or treatment with a vehicle control.