Figure 8.

ICAM1 confers a survival advantage to CCC cells. a) Western blot analysis of ICAM1 expression in OVISE cells transfected with shRNAs. N, normoxia for 24 h; H, 1% O₂ for 24 h. b) MTS assays revealed the viability of OVISE cells cultured under serum-free conditions. c) Relative survival rates of OVISE cells cultured as in b). The viabilities of cells cultured under SSH (estimated as in b) were normalized to viability rates observed with cells cultured under normoxia without FCS. Data shown are the mean (n = 3) ± SD. *P = 0.036; **P = 0.001; ***P = 0.009; ****P = 0.007. d) Left: western blot of PARP-1 in OVISE cells. N, normoxia for 72 h; H, 1% O₂ for 72 h. Right: caspase activities in OVISE cells cultured under SSH for 96 h.