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. Author manuscript; available in PMC: 2015 Apr 14.
Published in final edited form as: J Biotechnol. 2007 Oct 5;133(3):403–411. doi: 10.1016/j.jbiotec.2007.09.018

Fig. 3.

Fig. 3

Overexpression and purification of the LamA/SbpA fusion protein. (a) SDS-PAGE analysis after Coomassie staining. Lane 1, Bench mark ladder (All blue, Biorad laboratories, Hercules, CA); lane 2, E. coli BL21star(DE3) expression culture harboring plasmid pET28a.sbpA.lamA before induction; lane 3, E. coli BL21star(DE3) expression culture harboring plasmid pET28a.sbpA.lamA after 2 h of induction; lane 4, purified LamA/SbpA fusion protein. (b) Western blot analysis of LamA/SbpA using anti-vsv-g-peroxidase conjugate.