Fig. 4.

(a) Transmission electron micrograph of a negatively stained preparation of LamA/SbpA self-assembled in solution into a monomolecular array (bar, 100 nm). Inset showing the square lattice symmetry of the S-layer enzyme fusion protein with a lattice constant of 13.1 nm (enzyme moiety in blue); (b) fluorescent microscopic image of microspheres coated with LamA/SbpA after binding of an anti-vsv-g FITC conjugate (bar, 3 μm), indicating surface exposure of the enzymatic group; (c) AFM deflection image of the S-layer enzyme fusion protein after recrystallization on a silicon wafer, measured in contact mode in aqueous solution (bar, 100 nm); (d) electron micrograph of a porous membrane (bar, 1 μm) with a schematic representation of immobilized LamA/SbpA.