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. Author manuscript; available in PMC: 2015 Apr 14.
Published in final edited form as: J Biotechnol. 2007 Oct 5;133(3):403–411. doi: 10.1016/j.jbiotec.2007.09.018

Table 2.

Advantages and disadvantages of the S-layer fusion protein based enzyme immobilization system

Advantages
 No preceding surface activation of the support needed
 One-step incubation process
 Stabilization of the enzymatic group within the S-layer lattice
 S-layer cushion prevents denaturation of enzyme upon immobilization on solid supports
 Higher enzymatic activity per square unit
 Applicability to any functional group
 No complex optimization of immobilization conditions
Disadvantages
 Genetic engineering of the enzyme
 Refolding step needed