Stock concentration	Volume to prepare for
one mouse	five mice	ten mice
Ketamine	100 mg/ml	25 µl	125 µl	250 µl
Xylazine	100 mg/ml	2.5 µl	12.5 µl	25 µl
Isotonic saline		223 µl	1,113 µl	2,225 µl
Total	250 ul	1,250 µl	2,500 µl