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. 2015 Mar 31;2015:657325. doi: 10.1155/2015/657325

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Copyright © 2015 Zhe Geng et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Expression of exogenous RNAi-resistant TIAR rescues the function of TIAR in siRNA-treated cells. (a) Western blot analysis of the expression of TIAR and c-myc in control, TIAR RNAi, expressional control, and expressional TIAR. (b) Quantitative RT-PCR analysis of the gene expression of nestin, Pax6, Flk-1, brachyury, Sox17, and Gata6 on day 4 during EB differentiation in TIAR RNAi, expressional control, and expressional TIAR. (c) Western blot analysis of the gene expression of nestin, Flk-1, and Sox17 on day 4 during EB differentiation in expressional control and expressional TIAR. ^∗ P < 0.05 by one-way ANOVA. Control: E14 cells infected with an empty control pCDNA3.1-Hygro vector; TIAR RNAi: TIAR RNAi E14 cells; expressional control: TIAR RNAi E14 cells infected with an empty control pCDNA3.1-Hygro vector; expressional TIAR: TIAR RNAi E14 cells infected with pCDNA3.1-Hygro vector containing RNAi-resistant TIAR transgene.