Table 1.

Group definition and allocation of rats

		Euthanized at day 29			Euthanized at day 43
	H1PV dose (pfu)	Hematology Clinical biochemistry Urinalysis Histopathology	RT-PCR	Toxicokinetics	Hematology Clinical biochemistry Urinalysis Histopathology	Hematology Clinical biochemistry Urinalysis RT-PCR
Vehiclea	0	10 male	5 male	5 male	7 maleb	5 male
		10 female	5 female	5 female	6 femaleb	5 female
Ringer	0	5 male	none	none	5 male	none
		5 female	none	none	5 female	none
H1PV	106	10 male	5 male	5 male	none	none
		10 female	5 female	5 female	none	none
H1PV	107	10 male	5 male	5 male	none	none
		10 female	5 female	5 female	none	none
H1PV	108	10 male	5 male	5 male	5 male	5 male
		10 female	5 female	5 female	5 female	5 female

All rats received 750 µL per injection.

^aThe vehicle comprised 10% iodixanol in Ringer solution

^b2 additional male and one additional female rats were included, because 3 animals had to be euthanized prematurely (2 at day 15 and 1 at day 18) due to deteriorated health status unrelated to the vehicle administration. These additional animals were handled according to the same protocol as for the other rats in this group and received a total of 17 injections over 28 d.