Skip to main content
. 2015 Apr 14;13(4):e1002116. doi: 10.1371/journal.pbio.1002116

Fig 8. As SseB moves away from the Salmonella surface, it is associated with the vacuolar membrane.

Fig 8

(A) RAW264.7 macrophages were infected with WT Salmonella harboring pFPV25.1 for the constitutive expression of GFP (green). Cells were fixed, permeabilized, and immunostained for mouse monoclonal anti-LAMP-1 (blue) and rabbit polyclonal anti-SseB (red) followed by Alexa568- or Alexa647-labeled secondary antibodies, respectively, for various times as indicated. The merged image indicates that once SseB was detached from the bacterial cell surface, it was co-localized with the host endosomal membrane (i.e., LAMP-1–positive membranes). Images were obtained using the Nikon A1R confocal microscope. Localization resulted in magenta staining of Salmonella SseB as analyzed by Image J software. Scale bar = 3μm. (B) Results of three independent experiments in which macrophages were infected with WT GFP-expressing Salmonella and immunostained with LAMP-1 and SseB at 3.5 h, 4 h, 5 h, and 6 h post-infection. The values represent the mean ± SEM.