Fig 1. Schematic structural representation of the adapter and blocking oligo sequences and the required modifications.

Full-length sequences can be derived from the Illumina sequence letter [11]. Index = sequence of 6–8 nucleotides that makes each adapter unique, * = phosphothiorate bond, /invdT/ = inverted dT, /phos/ = phosphate group, rc. = reverse complement. Oligonucleotide sequences © 2007–2013 Illumina, Inc. All rights reserved. Derivative works created by Illumina customers are authorized for use with Illumina instruments and products only. All other uses are strictly prohibited.