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. 2015 Apr 14;10(4):e0125057. doi: 10.1371/journal.pone.0125057

Fig 3. Actin inhibitor (Latrunculin) induces apoptosis activation in cultured megakaryocytes.

Fig 3

A) FACS analysis of apoptosis activation by measuring Mitochondrial Outer Membrane Potential (MOMP) and phosphatidyl serine (PS) externalization B) Luminescence activity measuring Caspase 3 and 7 activity were measured using Caspase-Glo 3/7 Assay. Actin inhibition induces overexpression of both pro-apoptotic and pro-survival genes and increases BCL-XL protein synthesis C) RT-PCR analysis of mRNA expression of pro-apoptotic genes: BAX, BAK, BNIP3 and BNIP3L and pro-survival genes: BCL-2, BCL-XL, IGF1R and CFLAR relative to untreated control. BCL-XL protein showed increased synthesis with actin polymerization inhibitor treatment D) Western Blot (density ratio = 1.34 compared to untreated control) and E) Intracellular protein quantification with FACS. * p<0.05; ** p<0.005