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. 2015 Apr 14;10(4):e0123901. doi: 10.1371/journal.pone.0123901

Fig 4. Effect of siRNA-mediated knockdown for CDH1 in HCC4006ER cells.

Fig 4

(A) Total cell lysates were harvested 72 hours after reverse-transfection of negative control siRNA (siNC) or validated siRNAs for CDH1 which encodes E-cadherin mixed with Lipofectamine RNAiMAX. (B-D) Tumor cells were reverse-transfected at the same time plating into 96-wells and then incubated for 24 hours. They were incubated with various concentrations of docetaxel, paclitaxel and vinorelbine for additional 72 hours. Percent growth relative to DMSO-treated controls was evaluated by Cell Counting Kit-8 assay.