Fig 4. Exposure to Thomas-EMF inhibits B16-BL6 tumour growth in C57b mice.
A. A total of 35 male C57b mice (experiment 1 = 25 and experiment 2 = 10 animals) were injected subcutaneously with 106 B16-BL6 cells. The mice were randomly assigned to Thomas-EMF (red) or sham (grey) conditions and exposed for 3 h/day until the animals were sacrificed and tumours dissected. The average size and weight (and 95% CI and range) of the tumours are shown (* indicates p<0.05, Mann-Whitney U-test). B. The tumours from the mice in experiment 2 were fixed, embedded in paraffin, and sectioned for analysis for TUNEL and Ki-67 staining. Staining intensity for each section was assigned on a scale of 1–5 for each microscopic field. For analysis, 5 fields/tumour were scored for each mouse and the average (and 95% CI and range) of scores for 5 mice/condition were compared (* indicates p<0.05, Mann-Whitney U-test). Sections corresponding to average staining scores for each group are shown.