Table 1. List of differentially expressed proteins in B. subtilis due to curcumin treatment obtained from DIGE analysis and its comparison with iTRAQ analysis $ .
| Spot No | Uniprot ID | Name of the protein | Gene names | M.W (kDa) | No. of peptides | Fold change | iTRAQ (Orbitrap) | iTRAQ (QTOF) | ProteinScore | Ion Score | p-value |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 20 min curcumin treatment | |||||||||||
| D-1* | P37809 | ATP synthase subunit beta | AtpD | 51.42 | 19 | -1.56 | NS | NS | 662 | 423 | 0.032 |
| D-2 | P80698 | Trigger factor | Tig | 47.48 | 11 | -1.78 | -1.21 | -1.25 | 579 | 426 | 0.039 |
| U-1* | P46336 | Protein IolS | IolS | 35.17 | 13 | 1.85 | NS | 2.39 | 167 | 104 | 0.01 |
| U-2 | O31605 | Oligoendopeptidase F homolog | YjbG | 77.07 | 4 | 1.78 | 1.59 | 1.58 | 109 | 85 | 0.019 |
| 60 min curcumin treatment | |||||||||||
| D-1* | P37253 | Ketol-acid reductoisomerase | IlvC | 37.43 | 19 | -1.67 | NS | NI | 1240 | 1163 | 0.043 |
| D-2* | P42973 | Aryl-phospho-beta-D-glucosidase BglA | BglA | 54.84 | 18 | -1.69 | NS | NS | 266 | 229 | 0.04 |
| D-3 # | O32114 | Diaminopimelate epimerase | DapF | 30.85 | 4 | -1.72 | NI | NI | 109 | 100 | 0.0067 |
| D-4* | P20429 | DNA-directed RNA polymerase alpha chain | RpoA | 34.77 | 13 | -1.95 | NS | -1.42 | 245 | 200 | 0.029 |
| D-5* | P22250 | Glutamate—tRNA ligase | GltX | 55.72 | 36 | -2.23 | NS | NS | 783 | 648 | 0.026 |
| D-6 | Q07836 | Uncharacterized protein yxxG | YxxG | 16.43 | 4 | -2.72 | -1.61 | -5.00 | 274 | 207 | 0.027 |
| D-7 | P80868 | Elongation factor G | FusA | 76.62 | 50 | -5.01 | -1.45 | -1.48 | 1120 | 889 | 0.041 |
| D-8 | P80868 | Elongation factor G | FusA | 76.62 | 49 | -5.92 | -1.45 | -1.48 | 1090 | 884 | 0.014 |
| U-1 | O32201 | Protein LiaH | LiaH | 25.69 | 31 | 17 | 6.63 | 2.23 | 788 | 663 | 0.0017 |
| U-2* | P21464 | 30S ribosomal protein S2 | RpsB | 27.96 | 15 | 4.22 | NS | -1.34 | 601 | 534 | 0.00026 |
| U-3 | P49814 | Malate dehydrogenase | Mdh | 33.64 | 13 | 2.97 | 2.17 | 1.63 | 628 | 586 | 0.047 |
| U-4* | P80866 | Vegetative protein 296 | YurY | 29.03 | 9 | 2.95 | NS | NS | 110 | 96 | 0.035 |
| U-5* | P17820 | Chaperone protein dnaK | DnaK | 66 | 29 | 2.79 | NS | NS | 1000 | 793 | 0.0041 |
| U-6 | P54550 | Probable NADH-dependent flavin oxidoreductase yqiM | YqiM | 37.58 | 14 | 2.33 | 1.37 | 2.82 | 513 | 463 | 0.05 |
| U-7 | P80864 | Probable thiol peroxidise | Tpx | 18.31 | 10 | 2.18 | 1.56 | 1.52 | 303 | 233 | 0.0053 |
| U-8 | P96611 | Thioredoxin-like protein ydbP | YdbP | 12.43 | 5 | 2.11 | 1.39 | 1.39 | 120 | 102 | 0.032 |
| U-9* | P49778 | Elongation factor P | Efp | 20.47 | 7 | 2.07 | NS | NS | 203 | 175 | 0.032 |
| U-10* | P37809 | ATP synthase beta chain | AtpD | 51.42 | 29 | 1.96 | NS | NS | 1350 | 1192 | 0.045 |
| U-11 | O35022 | FMN-dependent NADH-azoreductase 1 | AzoR1 | 22.98 | 17 | 1.78 | 1.85 | NI | 436 | 391 | 0.036 |
| U-12 | P28599 | 10 kDa chaperonin | GroS | 10.17 | 5 | 1.51 | 1.81 | 1.65 | 157 | 122 | 0.038 |
| 120 min curcumin treatment | |||||||||||
| D-1 | P23630 | Diaminopimelate decarboxylase | LysA | 48.7 | 18 | -3.64 | -1.35 | -1.51 | 482 | 414 | 0.0034 |
| D-2 | P30950 | Delta-aminolevulinic acid dehydratase | HemB | 36.2 | 4 | -3.57 | -1.52 | -1.33 | 146 | 102 | 0.012 |
| D-3 | P32395 | Uroporphyrinogen decarboxylase | HemE | 39.64 | 3 | -3.57 | -1.29 | 1.62 | 208 | 164 | 0.039 |
| D-4 | P54419 | S-adenosylmethionine synthetase | MetK | 44 | 10 | -1.67 | -2.33 | -2.45 | 104 | 83 | 0.0079 |
| D-5* | P39121 | Deoxyribose-phosphate aldolase | DeoC | 22.19 | 6 | -2.35 | NS | NS | 175 | 150 | 0.0045 |
| D-6 | P05654 | Aspartate carbamoyltransferase | PyrB | 34.31 | 16 | -2.29 | -1.94 | NI | 468 | 373 | 0.028 |
| D-7 | P52996 | 3-methyl-2-oxobutanoate hydroxymethyltransferase | PanB | 30 | 3 | -1.94 | NS | -1.92 | 328 | 284 | 0.037 |
| D-8* | P21882 | Pyruvate dehydrogenase E1 component subunit beta | PdhB | 35.47 | 26 | -1.69 | NS | NS | 317 | 171 | 0.015 |
| D-9* | Q9KWU4 | Pyruvate carboxylase | Pyc | 127.9 | 30 | -2.75 | NS | NS | 315 | 188 | 0.0012 |
| D-10 | O32117 | NADH dehydrogenase-like protein yutJ | YutJ | 39.75 | 19 | -2.28 | -1.51 | NI | 865 | 737 | 0.014 |
| U-1 | P80244 | ATP-dependent Clp protease proteolytic subunit | ClpP | 21.6 | 5 | 1.63 | 2.26 | 2.35 | 323 | 241 | 0.05 |
| U-2* | P21880 | Dihydrolipoyl dehydrogenase | PdhD | 49.7 | 20 | 1.67 | NS | -1.24 | 996 | 929 | 0.024 |
| U-3* | P20429 | DNA-directed RNA polymerase alpha chain | RpoA | 34.77 | 13 | 2 | NS | -1.28 | 245 | 200 | 0.029 |
| U-4 | P81101 | Ribosome recycling factor | Frr | 20.62 | 10 | 2.56 | 1.50 | 1.56 | 346 | 273 | 0.047 |
| U-5 | O32259 | Lactate utilization protein C | LutC | 26.27 | 6 | 2.58 | 1.26 | NS | 223 | 199 | 0.02 |
| U-6* | P37487 | Manganese-dependent inorganic pyrophosphatase | PpaC | 34 | 4 | 2.6 | NS | NS | 379 | 283 | 0.04 |
| U-7 | P28598 | 60 kDa chaperonin | GroL | 57.25 | 25 | 2.84 | 2.10 | 1.95 | 1340 | 1224 | 0.048 |
| U-8 | P15874 | GrpE protein (HSP-70 cofactor) | GrpE | 21.53 | 6 | 3.98 | 1.50 | NS | 186 | 167 | 0.023 |
| U-9* | P37869 | Enolase | Eno | 46.42 | 8 | 5.86 | NS | NS | 75 | 49 | 0.0034 |
| U-10* | P39121 | Deoxyribose-phosphate aldolase | DeoC | 23.47 | 13 | 1.72 | NS | NS | 611 | 524 | 0.041 |
| U-11 | P09339 | Aconitate hydratase | CitB | 99.61 | 28 | 4.13 | 4.56 | 3.88 | 789 | 664 | 0.0015 |
$ This is a partial list having selected candidates with >1.5 fold change and complete list is provided in S2 Table
#: Proteins unique in DIGE, Bold: Same trend in both DIGE and iTRAQ (Orbitrap data);
* or NS: No significant change in iTRAQ in Orbitrap data (less than 1.2 fold up and down);
NI- Not identified in iTRAQ analysis.