Table 2. Partial list of differentially expressed proteins in B. subtilis due to curcumin treatment obtained from iTRAQ analysis*.
| UniProt ID | Name of the protein | Gene name | Coverage | Uni. Peptides (Orbitrap) | Fold change (Orbitrap) | Uni. Peptides (Q-TOF) | Fold change (QTOF) | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 20 min | 60 min | 120 min | 20 min | 60 min | 120 min | ||||||
| Cell wall synthesis | |||||||||||
| P70965 | UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1 | MurAA | 35.32 | 13 | 0.89 | 0.64 | 0.41 | 13 | 1.36 | 0.702 | 0.549 |
| Q03522 | UDP-N-acetylmuramoylalanine—D-glutamate ligase | MurD | 31.04 | 13 | 0.98 | 0.67 | 0.47 | 4 | 1.115 | 1.039 | 0.812 |
| P40778 | UDP-N-acetylmuramate—L-alanine ligase | MurC | 25.46 | 9 | 1.03 | 0.71 | 0.54 | 6 | 1.296 | 1.170 | 0.580 |
| Q03523 | UDP-N-acetylmuramoyl-L-alanyl-D-glutamate—2,6-diaminopimelate ligase | MurE | 37.04 | 14 | 1.02 | 0.74 | 0.55 | 9 | 0.840 | 0.773 | 0.379 |
| P37585 | UDP-N-acetylglucosamine—N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase | MurG | 37.47 | 11 | 0.91 | 0.8 | 0.63 | 3 | 0.693 | 0.682 | 0.462 |
| P94556 | Glutamate racemase 1 | RacE | 25.37 | 4 | 0.97 | 0.84 | 0.68 | 2 | 0.773 | 0.857 | 0.539 |
| P96613 | UDP-N-acetylmuramoyl-tripeptide—D-alanyl-D-alanine ligase | MurF | 30.20 | 8 | 1.10 | 0.79 | 0.87 | 3 | 1.721 | 0.934 | 0.846 |
| P0CI73 | Glutamine—fructose-6-phosphate aminotransferase [isomerizing] | GlmS | 30.33 | 15 | 0.92 | 0.79 | 0.53 | 10 | 0.891 | 0.761 | 0.535 |
| P14192 | Bifunctional protein GlmU | GlmU | 23.03 | 8 | 0.93 | 0.83 | 0.58 | 7 | 2.139 | 1.505 | 0.885 |
| Cell division and Sporulation | |||||||||||
| P45693 | Stage V sporulation protein S | SpoVS | 38.37 | 2 | 1.10 | 2.59 | 6.10 | 3 | 3.703 | 4.052 | 6.623 |
| P28015 | Putative septation protein SpoVG | SpoVG | 42.27 | 4 | 0.96 | 1.51 | 4.49 | 2 | 0.912 | 0.712 | 2.091 |
| Q07867 | Cell division protein FtsL | FtsL | 8.55 | 1 | 0.72 | 1.41 | 3.09 | NI | NI | NI | NI |
| P0CI74 | Cell cycle protein GpsB | GpsB | 43.88 | 4 | 0.98 | 1.06 | 1.46 | 2 | 1.173 | 1.452 | 1.772 |
| P06628 | Sporulation initiation phosphotransferase F | SpoOF | 23.39 | 2 | 0.79 | 1.75 | 2.93 | 2 | 0.566 | 1.686 | 2.371 |
| Q01368 | Stage III sporulation protein AB | SpoIIIAB | 5.26 | 1 | 0.89 | 1.29 | 2.02 | NI | NI | NI | NI |
| P06534 | Stage 0 sporulation protein A | SpoOA | 13.86 | 3 | 0.97 | 1.33 | 2.09 | 1 | 1.158 | ||
| P71088 | Sporulation-control protein spo0M | SpoOM | 37.21 | 10 | 1.23 | 1.00 | 1.61 | 5 | 1.446 | 0.986 | 0.877 |
| P39624 | Spore coat polysaccharide biosynthesis protein SpsD | SpsD | 4.84 | 1 | 0.97 | 0.43 | 1.13 | NI | NI | NI | NI |
| P37470 | Peptidyl-tRNA hydrolase | SpoVC | 13.83 | 2 | 1.10 | 0.82 | 0.61 | 2 | 2.023 | 0.587 | 0.877 |
| Fatty acid synthesis | |||||||||||
| O34746 | 3-oxoacyl-[acyl-carrier-protein] synthase 3 protein 1 | FabHA | 45.83 | 13 | 1.01 | 0.56 | 0.38 | 6 | 0.914 | 0.584 | 0.438 |
| O07600 | 3-oxoacyl-[acyl-carrier-protein] synthase 3 protein 2 | FabHB | 18.15 | 6 | 0.94 | 0.54 | 0.41 | 2 | 1.079 | 0.407 | 0.480 |
| P71019 | Malonyl CoA-acyl carrier protein transacylase | FabD | 48.26 | 14 | 0.93 | 0.64 | 0.44 | 13 | 0.976 | 0.620 | 0.564 |
| P54616 | Enoyl-[acyl-carrier-protein] reductase [NADH] FabI | FabI | 51.16 | 11 | 0.91 | 0.65 | 0.54 | 6 | 0.975 | 0.905 | 0.787 |
| O34340 | 3-oxoacyl-[acyl-carrier-protein] synthase 2 | FabF | 48.67 | 14 | 1.03 | 0.73 | 0.57 | 11 | 1.146 | 0.858 | 0.818 |
| P51831 | 3-oxoacyl-[acyl-carrier-protein] reductase FabG | FabG | 61.79 | 12 | 1.01 | 0.71 | 0.58 | 7 | 1.318 | 0.912 | 0.794 |
| Stress response | |||||||||||
| P37571 | Negative regulator of genetic competence ClpC/MecB | ClpC | 59.88 | 45 | 1.20 | 1.65 | 2.43 | 29 | 1.264 | 1.809 | 2.746 |
| P80244 | ATP-dependent Clp protease proteolytic subunit | ClpP | 43.15 | 8 | 1.09 | 2.07 | 2.26 | 4 | 1.283 | 2.136 | 2.208 |
| P39778 | ATP-dependent protease ATPase subunit ClpY | ClpY | 35.55 | 14 | 1.17 | 1.41 | 1.82 | 5 | 1.835 | 1.589 | 2.231 |
| O31673 | ATP-dependent Clp protease ATP-binding subunit ClpE | ClpE | 16.17 | 5 | 0.95 | 1.72 | 1.76 | NI | NI | NI | NI |
| P39070 | ATP-dependent protease subunit ClpQ | ClpQ | 21.55 | 4 | 1.13 | 1.08 | 1.61 | NI | NI | NI | NI |
| P54617 | Phage shock protein A homolog | YdjF | 58.59 | 11 | 0.76 | 1.22 | 3.09 | 6 | 1.065 | 1.472 | 2.324 |
| P54375 | Superoxide dismutase [Mn] | SodA | 67.82 | 9 | 1.11 | 1.59 | 2.69 | 6 | 1.148 | 1.224 | 2.161 |
| P42297 | Universal stress protein YxiE | YxiE | 45.27 | 5 | 1.19 | 0.95 | 2.34 | 3 | 0.901 | 0.859 | 1.609 |
| P51777 | Cold shock protein CspD | CspD | 89.39 | 5 | 0.92 | 1.22 | 2.25 | 3 | 0.920 | 1.452 | 1.325 |
| P28599 | 10 kDa chaperonin | GroS | 73.40 | 7 | 1.09 | 1.82 | 2.20 | 5 | 1.329 | 1.824 | 2.662 |
| P28598 | 60 kDa chaperonin | GroL | 74.63 | 39 | 1.07 | 1.69 | 2.10 | 24 | 1.187 | 1.885 | 2.289 |
| P39158 | Cold shock protein CspC | CspC | 59.09 | 5 | 0.22 | 0.82 | 1.86 | 1 | 0.304 | 0.863 | 1.842 |
| P81100 | Stress response protein SCP2 | YceC | 37.69 | 6 | 1.05 | 1.30 | 1.74 | 2 | 1.381 | 1.308 | 2.002 |
| P54377 | Probable glycine dehydrogenase [decarboxylating] subunit 2 | GcvPB | 9.43 | 3 | 1.20 | 2.15 | 1.67 | 1 | 0.693 | 2.848 | 2.690 |
| O32221 | Copper chaperone CopZ | CopZ | 68.12 | 3 | 0.66 | 1.04 | 1.63 | 1 | 1.427 | 0.873 | |
| P15874 | Protein GrpE | GrpE | 57.75 | 10 | 0.96 | 1.13 | 1.51 | 5 | 1.399 | 1.455 | 1.497 |
| P80875 | General stress protein 16U | YceD | 49.74 | 6 | 1.05 | 1.30 | 1.50 | 6 | 1.085 | 1.542 | 1.672 |
| TCA cycle | |||||||||||
| P39120 | Citrate synthase 2 | CitZ | 31.18 | 11 | 1.20 | 3.99 | 7.65 | 7 | 1.621 | 3.706 | 8.131 |
| P09339 | Aconitate hydratase | CitB | 33.22 | 25 | 1.14 | 3.94 | 4.56 | 12 | 2.032 | 4.297 | 4.724 |
| P39126 | Isocitrate dehydrogenase [NADP] | Icd | 44.21 | 20 | 1.10 | 3.06 | 3.53 | 9 | 2.008 | 2.694 | 3.139 |
| P23129 | 2-oxoglutarate dehydrogenase E1 component | OdhA | 35.28 | 27 | 1.37 | 2.90 | 1.96 | 14 | 1.286 | 2.223 | 1.744 |
| P16263 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex | OdhB | 54.44 | 18 | 1.18 | 2.51 | 3.30 | 13 | 2.203 | 4.063 | 3.053 |
| P80865 | Succinyl-CoA ligase [ADP-forming] subunit alpha | SucD | 33.00 | 6 | 1.12 | 2.73 | 3.39 | 4 | 1.553 | 2.023 | 3.060 |
| P80886 | Succinyl-CoA ligase [ADP-forming] subunit beta | SucC | 55.32 | 22 | 1.11 | 3.26 | 4.08 | 15 | 1.252 | 5.002 | 4.043 |
| P08065 | Succinate dehydrogenase flavoprotein subunit | SdhA | 49.32 | 22 | 1.44 | 2.40 | 2.06 | 9 | 1.061 | 2.320 | 2.220 |
| P08066 | Succinate dehydrogenase iron-sulfur subunit | SdhB | 27.27 | 6 | 1.33 | 2.14 | 1.71 | 3 | 1.347 | 2.216 | 1.813 |
| Nucleotide biosynthesis | |||||||||||
| P29726 | Adenylosuccinate synthetase | PurA | 26.05 | 10 | 1.12 | 0.76 | 0.55 | 7 | 1.189 | 0.814 | 1.119 |
| P12047 | Adenylosuccinate lyase | PurB | 35.27 | 15 | 0.95 | 0.77 | 0.45 | 4 | 0.966 | 0.722 | 0.535 |
| P12046 | Phosphoribosylaminoimidazole-succinocarboxamide synthase | PurC | 4.98 | 1 | 0.61 | 0.95 | 0.62 | NI | NI | NI | NI |
| P12039 | Phosphoribosylamine—glycine ligase | PurD | 12.56 | 4 | 0.72 | 0.91 | 0.62 | NI | NI | NI | NI |
| P12044 | N5-carboxyaminoimidazole ribonucleotide mutase | PurE | 19.75 | 2 | 0.81 | 1.02 | 0.57 | 2 | 0.740 | 0.761 | 1.417 |
| P12048 | Bifunctional purine biosynthesis protein PurH | PurH | 27.54 | 11 | 0.66 | 0.98 | 0.56 | 5 | 0.491 | 1.212 | 0.584 |
| P12042 | Phosphoribosylformylglycinamidine synthase 2 | PurL | 3.10 | 2 | 0.55 | 0.82 | 0.53 | NI | NI | NI | NI |
| P29727 | GMP synthase [glutamine-hydrolyzing] | GuaA | 58.67 | 27 | 1.03 | 0.64 | 0.42 | 17 | 1.162 | 0.714 | 0.538 |
| O05269 | GMP reductase | GuaC | 48.16 | 10 | 1.06 | 1.00 | 0.49 | 4 | 1.766 | 2.612 | 1.020 |
| P14193 | Ribose-phosphate pyrophosphokinase | Prs | 35.65 | 11 | 0.93 | 0.74 | 0.52 | 8 | 0.889 | 0.884 | 0.707 |
* This is a partial list having selected candidates; complete list is provided in S2 Table
NI- Not identified in Q-TOF