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| Gene | Forward primer (5'-3') | Reverse primer (5'-3') | Annealing temperature (°C) | Amplicon size (bp) |
|---|---|---|---|---|
| MurAA | 5'TACAGGTCATGCAAGAGT-3' | 5'TTCTCTGTAGCTCCTACACT-3' | 46 | 196 bp |
| FtsH | 5'CACCGTTATCGGTCTCGTTT-3' | 5'CCAAGAGGCCGACAATTTTA-3' | 46 | 158 bp |
| SpoVG | 5'TTCGTGTGATTGATGGAAACA-3' | 5'TGCTTCAGTGTCACCCAGAC -3' | 46 | 164 bp |
| 16S r RNA | 5'GATCTTAGTTGCCAGCATTC-3' | 5'TTACTAGCGATTCCAGCTTC-3' | 46 | 233 bp |
