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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Neurochem. 2015 Jan 26;133(3):452–464. doi: 10.1111/jnc.13022

Fig. 3.

Fig. 3

Estimations of ChAT positive motor neurons in the spinal cords of monogenic and bigenic transgenic mice. Randomly selected spinal cord tissue sections from different levels of the cord were stained with ChAT antibody to identify motor neurons of the ventral horn. The number of ChAT immunoreactive motor neurons in spinal sections from at least 2–3 slides per animal, with each slide containing 2–4 spinal cord coronal sections, were counted by two people independently that were blind to genotypes. Data were statistically analyzed as described in Materials and Methods (see Results for p values). The brackets identify data sets that were determined to be statistically different (marked by *). The numbers of mice analyzed for each genotype were as follows: NTg (n=8), Z104 (n=15), hSOD1-L126Z (Line45) (n=9), bigenic L45/αB-crys (n=9), hSOD1-G93A (n=5), bigenic G93A/αB-crys (n=10). The error bar notes the standard error of the mean (SEM).