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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Neurochem. 2015 Jan 26;133(3):452–464. doi: 10.1111/jnc.13022

Fig. 7.

Fig. 7

Co-expression of αB-crys with mutant SOD1 does not specifically diminish the amount of slowly migrating SOD1 immunoreactivity in BN-gels. HEK 293FT cells were transiently transfected with WT or mutant SOD1 alone or in co-transfection with vectors to express αB-crys. Immunoblots of BN-gels of cell lysates were probed with the hSOD1 antibody. Portions of the same lysates were analyzed by SDS-PAGE and immunoblotting with the hSOD1 antibody. Co-expression of αB-crys appeared to reduce the intensity of SOD1 reactivity on BN-gels (A and B), but did not collapse the oligomeric immunoreactive species into a species migrating as the expected size for monomeric L126Z-hSOD1, and the difference in total mutant SOD1 immunoreactivity between Line 45 and Lin 45 × Z104 mice was not statistically significant (Students’ s t-Test). The error bar notes the standard deviation (SD).