Figure 5.

Nocifensive behavior in rats after administration of ET-1 or BK. Two intraplantar injections were administered in 5 min intervals. First injection consisted of 25 µl of PyR3 (100 nM), Gd³⁺ (100 nM), or PBS as vehicle. Second contained 25 µl ET-1 (10 nM) and BK (10 nM). Nocifensive behavior was recorded throughout the time course of experiment (40 min) and quantified every 5 min. (a) Summary of ET-1-induced behavior in rats in the presence or absence of ion channel inhibitor injected 5 min prior to peptide injection. (b) Summary of BK-induced behavior in rats in the presence or absence of ion channel inhibitor, injected 5 min prior to peptide injection. Statistical significance was assessed by two-way ANOVA with Bonferroni correction, n = 6–9, *p < .05. **p < .01. ***p < .001. ET-1 = endothelin-1; BK = bradykinin; PBS = phosphate buffered saline; ANOVA = analysis of variance.