. 2014 Mar 19;52(5):2878–2885. doi: 10.1007/s13197-014-1319-5

Table 2.

Effect of phase composition in ILs–salt ATPS on purification of wheat-esterase ( $\bar{x}$ ± SD, n = 3)

Salts in ATPS(w/w)	V _R	K _P	K _E	Y(%)	PF
NaH₂PO₄15 %	1.55 ± 0.04^d*	0.73 ± 0.00^abc	1.12 ± 0.03^b	63.55 ± 0.32^c	1.44 ± 0.01^e
NaH₂PO₄20 %	1.44 ± 0.01^d	0.64 ± 0.02^ab	1.86 ± 0.01^c	72.80 ± 0.09^e	2.07 ± 0.01ⁱ
NaH₂PO₄25 %	1.11 ± 0.02^c	0.72 ± 0.02^abc	7.27 ± 0.06^h	88.93 ± 0.12ⁱ	4.23 ± 0.04^j
NaH₂PO₄30 %	1.07 ± 0.24^bc	1.15 ± 0.04^cd	8.62 ± 0.09ⁱ	89.98 ± 1.86ⁱ	1.97 ± 0.04^h
K₂HPO₄15 %	1.11 ± 0.01^c	1.18 ± 0.07^cd	3.29 ± 0.08^e	78.48 ± 0.50^g	2.14 ± 0.13ⁱ
K₂HPO₄20 %	1.82 ± 0.05^e	2.05 ± 0.02^e	4.72 ± 0.29^f	89.56 ± 0.36ⁱ	1.39 ± 0.05^e
K₂HPO₄25 %	1.12 ± 0.07^c	4.99 ± 0.09^fg	5.02 ± 0.03^f	84.89 ± 0.83^h	1.28 ± 0.06^d
K₂HPO₄30 %	0.88 ± 0.01^b	4.61 ± 0.04^f	2.43 ± 0.02^d	68.23 ± 0.22^d	0.85 ± 0.04^a
(NH₄)₂SO₄15 %	3.62 ± 0.25^g	1.02 ± 0.02^abcd	0.62 ± 0.02^a	69.16 ± 2.05^d	0.89 ± 0.03^a
(NH₄)₂SO₄20 %	1.57 ± 0.19^d	1.11 ± 0.03^bcd	1.89 ± 0.04^c	74.65 ± 2.02^ef	1.13 ± 0.04^c
(NH₄)₂SO₄25 %	0.86 ± 0.08^b	2.20 ± 0.52^e	1.81 ± 0.04^c	60.81 ± 2.68^b	1.01 ± 0.01^b
(NH₄)₂SO₄30 %	0.61 ± 0.07^a	5.14 ± 0.87^g	6.15 ± 0.90^g	78.64 ± 2.49^g	1.12 ± 0.03^c
MgSO₄15 %	---	---	---	---	---
MgSO₄20 %	2.98 ± 0.20^f	1.10 ± 0.05^bcd	1.09 ± 0.02^b	76.45 ± 1.48^fg	0.94 ± 0.05^ab
MgSO₄25 %	1.17 ± 0.03^c	0.58 ± 0.03^a	1.76 ± 0.06^c	67.20 ± 0.69^d	1.72 ± 0.07^g
MgSO₄30 %	0.88 ± 0.01^b	1.33 ± 0.03^d	0.77 ± 0.02^ab	40.60 ± 0.68^a	1.62 ± 0.07^f

V _R volume ratio, K _P protein distribution coefficient, K _E enzyme distribution coefficient, PF purified fold, Y yield

*a, b, c,……, j means within a row not sharing a common superscript differ significantly (p < 0.05)

--- no ATPS formed