Table 3.
Effect of pH in [Bmim]BF4–NaH2PO4 ATPS on purification of wheat-esterase ( ± SD, n = 3)
pH | V R | K P | K E | Y(%) | PF |
---|---|---|---|---|---|
4.8 | 1.11 ± 0.02a | 0.72 ± 0.02a | 7.27 ± 0.06e | 88.93 ± 0.12d | 4.23 ± 0.04d |
6.0 | 1.20 ± 0.02c | 1.18 ± 0.07b | 5.56 ± 0.14d | 86.94 ± 0.06c | 3.29 ± 0.20c |
7.0 | 1.38 ± 0.03b | 1.57 ± 0.02c | 4.86 ± 0.07c | 87.05 ± 0.11c | 2.03 ± 0.07b |
8.0 | 1.12 ± 0.07a | 2.18 ± 0.03d | 4.33 ± 0.13b | 82.89 ± 0.73b | 2.18 ± 0.03b |
9.0 | 1.19 ± 0.02b | 4.61 ± 0.04e | 2.96 ± 0.05a | 77.89 ± 0.12a | 1.50 ± 0.04a |
V R volume ratio, K P protein distribution coefficient, K E enzyme distribution coefficient, PF purified fold, Y yield
*a, b, c, d means within a row not sharing a common superscript differ significantly (p < 0.05)