. 2014 Mar 19;52(5):2878–2885. doi: 10.1007/s13197-014-1319-5

Table 4.

Effect of temperature in [Bmim]BF₄–NaH₂PO₄ ATPS on purification of wheat-esterase ( $\bar{x}$ ± SD, n = 3)

Temperature °C	V _R	K _P	K _E	Y(%)	PF
10	1.03 ± 0.01^a	0.76 ± 0.01^b	7.33 ± 0.05^b	88.35 ± 0.07^a	4.18 ± 0.02^ab
20	1.10 ± 0.01^b	0.71 ± 0.01^a	7.23 ± 0.04^a	88.81 ± 0.12^b	4.25 ± 0.03^b
30	1.11 ± 0.02^b	0.72 ± 0.02^a	7.27 ± 0.06^ab	88.93 ± 0.12^b	4.23 ± 0.04^b
40	1.13 ± 0.01^c	0.75 ± 0.00^ab	7.22 ± 0.05^a	89.12 ± 0.06^c	4.18 ± 0.04^ab
50	1.18 ± 0.01^d	0.75 ± 0.00^ab	7.24 ± 0.01^a	89.51 ± 0.11^d	4.23 ± 0.04^b

V _R volume ratio, K _P protein distribution coefficient, K _E enzyme distribution coefficient, PF purified fold, Y yield

*a, b, c, d means within a row not sharing a common superscript differ significantly (p < 0.05)