Twelve percent SDS-PAGE of Ganoderma lucidum protein extracts digested with pronase. a crude mycelia protein extract, lane 1: molecular weight markers; lane 2: pronase 4 μg; lane 3: crude mycelia protein extract; lane 4; 6; 8: control sample at 30 min, 1 h, 2 h; lane 5; 7; 9: digested sample at 30 min, 1 h and 2 h, respectively, b crude fruiting bodies protein extract digested with pronase. Lane 1: molecular weight markers, lane 2: pronase in the reaction, lane 3; 5; 7: control 30 min, 1 h, 2 h, lane 4, 6, 8: treated with pronase at 40 °C for 30 min, 1 and 2 h respectively