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. 2014 Apr 23;52(5):2974–2981. doi: 10.1007/s13197-014-1355-1

Table 2.

The coded and corresponding real values of experimental parameters and their actual responses in central composite design

Standard order Run Factor 1 Factor 2 Factor 3 Response 1 Response 2 Response 3 Response 4 Yield
Microwave power (W) Irridation Time (min) Acetone:H2O (%) TPCa FRAPa DPPHb TACc mg/100 g dry weight
1 13 300 (−1) 6 (−1) 25 (−1) 1.096 0.122 92.859 0.188 0.21
2 18 500 (1) 6 (−1) 25 (−1) 0.951 0.085 98.697 0.168 0.22
3 14 300 (−1) 10 (1) 25 (−1) 0.984 0.109 96.089 0.178 0.22
4 9 500 (1) 10 (1) 25 (−1) 0.996 0.104 99.543 0.168 0.22
5 15 300 (−1) 6 (−1) 75 (1) 0.328 0.068 70.652 0.063 0.13
6 11 500 (1) 6 (−1) 75 (1) 0.463 0.089 81.448 0.086 0.15
7 19 300 (−1) 10 (1) 75 (1) 0.385 0.051 79.047 0.077 0.13
8 2 500 (1) 10 (1) 75 (1) 0.537 0.105 88.815 0.098 0.16
9 16 200 (−2) 8 (0) 50 (0) 0.674 0.077 99.433 0.120 0.16
10 5 600 (2) 8 (0) 50 (0) 0.809 0.093 99.870 0.131 0.19
11 1 400 (0) 4 (−2) 50 (0) 0.745 0.077 64.407 0.114 0.18
12 4 400 (0) 12 (2) 50 (0) 0.778 0.085 73.979 0.136 0.20
13 8 400 (0) 8 (0) 0 (−2) 1.012 0.106 95.012 0.195 0.24
14 20 400 (0) 8 (0) 100 (2) 0.028 0.054 61.306 0.021 0.10
15 3 400 (0) 8 (0) 50 (0) 0.801 0.094 86.855 0.107 0.17
16 17 400 (0) 8 (0) 50 (0) 0.804 0.107 89.916 0.142 0.17
17 7 400 (0) 8 (0) 50 (0) 0.796 0.095 87.384 0.107 0.19
18 12 400 (0) 8 (0) 50 (0) 0.783 0.103 89.688 0.134 0.18
19 10 400 (0) 8 (0) 50 (0) 0.804 0.110 88.970 0.133 0.19
20 6 400 (0) 8 (0) 50 (0) 0.854 0.095 88.298 0.143 0.18

a All data were expressed as mg tannic acid equivalent per gram dry weight algae

b All data were expressed as Radical scavenging activity (%)

c All data were expressed as mg ascorbic acid equivalent per gram dry weight algae