Fig. 2. Effect of delphinidin on extracellular matrix production induced by TGF-β1 in NPDFs. NPDFs were treated with TGF-β1 (5 ng/mL), with or without delphinidin (Del, 0-20 µM). The mRNA expression levels of collagen type I and fibronectin were determined by RT-PCR (A). The protein expression level of fibronectin was measured by Western blot (B), and the total amount of collagen was measured by Sircol assay (C). Values are the means ±SEM of the 3 independent experiments. ^*P<0.05 vs control; ^†P<0.05 vs TGF-β1.