Table 1. Proportions of Follicles with Good Morphology (Grade I) and Apoptotic Follicles According to Supplementation of Antifreeze Protein III in Vitrification Solution after Warming.
| Control | AFP 5 mg/mL | AFP 20 mg/mL | p value | |
|---|---|---|---|---|
| Number of ovaries | 12 | 14 | 12 | |
| Grade I follicles (%) | ||||
| Primordial | 18/49 (36.7)a | 39/90 (43.3)a | 31/53 (58.5)b | 0.071 |
| Primary | 18/58 (31.0)a | 26/42 (61.9)b | 32/75 (42.7)a | 0.009 |
| Secondary | 32/119 (26.9)a | 43/112 (38.4)a | 38/109 (34.9)a | 0.163 |
| Antral | 12/51 (23.5)a | 16/49 (32.7)a | 25/45 (55.6)b | 0.004 |
| Total | 80/277 (28.9)a | 124/293 (42.3)b | 126/282 (44.7)b | <0.001 |
| Apoptotic follicles (%) | 89/334 (26.6)a | 64/342 (18.7)b | 41/326 (12.6)c | <0.001 |
AFP, antifreeze protein.
a, b, c: different characters indicate significant difference by post-hoc analysis.