Table 2. Proportions of Follicles with Good Morphology (Grade I) and Apoptotic Follicles According to Supplementation of Antifreeze Protein III in Vitrification Solution after Autotransplantation of Vitrified-Warmed Ovary.
| Control | AFP 5 mg/mL | AFP 20 mg/mL | p value | |
|---|---|---|---|---|
| Number of ovaries | 37 | 38 | 25 | |
| Grossly intact ovaries (%) | 26/37 (70.3)a | 30/38 (78.9)a | 22/25 (88.0)a | 0.251 |
| Grade I follicles (%) | ||||
| Primordial | 37/94 (39.4)a | 55/99 (55.6)b | 69/93 (74.2)c | <0.001 |
| Primary | 30/64 (46.9)a | 31/78 (39.7)a | 22/38 (57.9)a | 0.182 |
| Secondary | 33/52 (63.5)a | 31/49 (63.3)a | 50/63 (79.4)a | 0.096 |
| Antral | 3/6 (50.0)a | 8/8 (100.0)a,b | 17/18 (94.4)b | 0.008 |
| Total | 103/216 (47.7)a | 125/234 (53.4)a | 158/212 (74.5)b | <0.001 |
| Apoptotic follicles (%) | 11/132 (8.3)a | 9/115 (7.8)a | 8/107 (7.5)a | 0.970 |
AFP, antifreeze protein.
a, b, c: different characters indicate significant difference by post-hoc analysis.