Fig. 1. Hypoxia decreases the levels of ZO-1 and E-cadherin in NHNE cells. (A) NHNE cells were incubated under hypoxic conditions, and Western blotting was performed to assess the expressions of HIF-1α, ZO-1, and E-cadherin at 8 and 16 hours of hypoxia. (B) The relative band intensities of ZO-1 and E-cadherin after 8 hours of hypoxia were calculated and normalized to β-actin. (C) NHNE cells were incubated under hypoxic conditions for 8 hours, and real-time PCR analysis was performed to compare the mRNA expressions of ZO-1 and E-cadherin. The relative gene expression was calculated by normalization to β-actin (n=3, ^*p<0.05, ^†p<0.01). HIF, hypoxia-inducible factor; NHNE, normal human nasal epithelial; PCR, polymerase chain reaction.