Fig. 2. Immunofluorescence analysis of NHNE cells. (A) NHNE cells were incubated under hypoxic conditions for 8 hours, and the immunofluorescence assay was performed with ZO-1 and E-cadherin antibodies. Fluorescence images were taken using a confocal microscope. (B) The mean fluorescence intensity was calculated using the ImageJ program (n=3, *p<0.05). NHNE, normal human nasal epithelial.
